The Walker Flat river crossing will be closed for two days next week as the last of South Australia's wooden-hulled ferries is taken out of service.
The crossing will be out of action between 9am on Tuesday and 4pm on Wednesday, weather permitting.
The nearest alternative crossings will be at Swan Reach and Mannum.
When the service reopens, it will be with the steel-hulled ferry Boobook, the last vessel of a fleet of four to be brought into service.
The new ferries were built by Bowhill Engineering and finished by the Department of Planning, Transport and Infrastructure at Morgan.
The $12.8 million ferry replacement program took four years to complete.
It became necessary after load restrictions needed to be introduced on the old wooden ferries as they aged.
Restrictions remain at just one of South Australia's 12 ferry crossings.
The ferry system has helped road freight, commuters, tourists and other road users to cross the River Murray since 1839.