The Tour Down Under will roll down the River Murray for the first time since 2011 this summer.
Stage four of the men's race will run from Norwood to Murray Bridge, via Palmer and Mannum, on January 24, 2020.
The second stage of the women's race will start on Murray Bridge's main street on January 17 and head up the hill to Birdwood.
But the biggest drawcard for visitors may be the 2020 Challenge Tour, the public road race, which is expected to bring about 6000 cyclists to Murray Bridge when it finishes there on January 24.
Mayor Brenton Lewis described the route announcement as a coup for the city, and one its sports-mad residents would embrace.
"The Rural City of Murray Bridge is excited to welcome the Santos Tour Down Under back to the region," he said.
"The TDU in Murray Bridge will be spectacular.
"I personally invite all residents and their families to come out and line the streets to welcome the riders, enjoy the free entertainment and what is sure to be two amazing days out."
Men's race director Mike Turtur said cycling fans would be entertained as Murray Bridge hosted a stage finish for the second time in the tour's 20-year history.
"I am expecting a battle to the end, as the crosswind the cyclists can expect to encounter between Mannum and Murray Bridge will be a considerable element to content with," he said.
"We know the fans will see some great cycling, as these conditions will suit the sprinters."
Competitors will climb from Norwood through the Adelaide Hills, including Mount Pleasant, Birdwood, Mount Torrens and Tungkillo, before rolling down through Palmer and Mannum to Murray Bridge.
Women's tour director Kimberley Conte said she was excited to see how the public would get involved with the event.
Italy's Fabio Sacchi claimed line honours the last time Murray Bridge hosted a stage finish in the men's Tour Down Under, in 2001.
Mannum, Tailem Bend and Strathalbyn have all since hosted stage starts or finishes.
The women's tour passed through Murray Bridge in 2015, the year before it gained international status, but was not widely advertised to the public.
- More information: www.tourdownunder.com.au.